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Image Search Results
Journal: Cells
Article Title: Global PIEZO1 Gain-of-Function Mutation Causes Cardiac Hypertrophy and Fibrosis in Mice
doi: 10.3390/cells11071199
Figure Lengend Snippet: PIEZO1 gain-of-function mutation causes cardiomyocyte hypertrophy and myocardial fibrosis. ( a ) WGA (wheat germ agglutinin) stained heart cross-sections of the PIEZO1 WT/WT and PIEZO1 M-R/M-R mice. Scale bars = 50 μm. ( b ) Quantitative analysis of total fibrotic area and ( c ) cardiomyocyte cross-sectional area using images of the type shown in ( a ). ( d – m ) Quantitative PCR mRNA expression data for Anp , Bnp , Acta1 , α-MHC , β-MHC , Col1a1 , Col3a1 , Ctgf , Serca2a and Piezo1 genes in whole heart of PIEZO1 WT/WT and PIEZO1 M-R/M-R mice. mRNA levels were normalised to reference gene expression and presented as fold change relative to PIEZO1 WT/WT mice. Data are for n = 9 PIEZO1 WT/WT and 9 PIEZO1 M-R/M-R mice for ( a – c ); n = 21–23 PIEZO1 WT/WT and 18–21 PIEZO1 M-R/M-R mice for ( d – j , l , m ); n = 15 PIEZO1 WT/WT and 12 PIEZO1 M-R/M-R mice for ( k ) (mean ± S.D.). Superimposed dots represent the individual values for each animal. # p < 0.05; ## p < 0.01 vs. PIEZO1 WT/WT mice. Unpaired Student’s t -test was used to evaluate statistical significance.
Article Snippet: After 1 h of blocking with 1% BSA in PBS, heart sections were stained at RT for 1 h with
Techniques: Mutagenesis, Staining, Real-time Polymerase Chain Reaction, Expressing